Concerns grew over the potential economic impact of the Ebola outbreak in west Africa after the World Bank warned that the region faced a $32.6bn economic hit if the disease were not contained.

This was the grimmest estimate yet of the economic cost of an outbreak that has left 3,400 people dead in west Africa and this week saw a nurse in Madrid catch the virus, the first case to originate outside Africa.

The warning came as doctors in Texas said a 42-year-old man who contracted Ebola in Liberia last month died at a Dallas hospital. In Spain, Mariano Rajoy, prime minister, appealed for calm after another nurse who was in contact with Ebola patients at a hospital in Madrid became the sixth person to be held under close medical surveillance there.